Interphase FISH Analysis for Translocations in Lymphomas
Histopathology Overview / Directory
Purpose of the test
Fluorescent in situ hybridisation analysis is recommended for the detection of translocations involving the BCL2, CCND1 (cyclin D1/BCL1), MYC, BCL6, ALK1 and MALT1 loci, detected in lymphomas. It has been demonstrated as invaluable for the diagnosis of lymphomas especially in small samples such as core biopsies and FNAs. This can be carried out in paraffin processed tissue and in conjunction with conventional immunohistochemical staining for these three proteins.
Sample Requirements
Formalin fixed paraffin processed tissue which ideally has not been decalcified in acid.
Storage and Transport
First class post within 4 days (temperature not to exceed 30°C during transport)
Turnaround Time
1 – 2 weeks
Price
Price available on application - please contact adrianturner1@nhs.net. Discounts could be available for significant workloads.
Contacts
Jane Moorhead
T 020 3299 3091
Lok Lam T 020 3299 9000 x4168
For clinical advice:
Dr S Pomplun T 020 3299 3190
Dr J Salisbury T 020 3299 3093
References
Pegoraro l. et al 1984. A 14:18 and an 8:14 translocation in a cell line derived from an acute B-cell leukaemia. PNAS 81: 9913-7.
Inaba T. et al. 1992 Genomic organization, chromosomal localization and independent expression of human cyclin D genes. Genomics 13: 565-74.
Hecht J and Aster J 2000. Molecular biology of Burkitt’s lymphoma. JCO 18: 3707-21.
