Autoantibodies (ASM, LKM, MITO, GPC)
Immunology and Allergy Overview / Directory
Purpose of the test
Our routine autoantibody screening is performed on the traditional rat liver, kidney and stomach substrate. This screening test is able to detect anti-smooth muscle antibodies (ASM), anti-mitochondrial antibodies (AMA), anti-liver kidney microsomal antibody (LKM) and anti-gastric parietal cell antibody (GPC). Anti-nuclear antibody (ANA) is also detectable but we recommend a HEp 2010 or HEp 2 substrate for investigating ANA. Patterns seen in this indirect immunofluorescence technique are subject to variability due to fixation of the substrate and other factors. The scientists performing this investigation are experienced fluorescence microscopists and may suggest further antigen specific testing in cases of atypical patterns. Titration is performed as a matter of routine.
Sample Requirements
2 ml freshly separated serum
Storage and Transport
Serum stored at 4°C should be sent by first class post
Turnaround Time
2 working days
Time Limit for Extra Tests
Five days
Factors affecting results or interpretation
All tests are compromised by prolonged transit times. For this reason, date of bleed and date of postage must be provided with each request.
Price
Price available on application - please contact adrianturner1@nhs.net. Discounts could be available for significant workloads.
Contacts
Dr Ted Davies, Consultant Clinical Scientist
T 020 3299 9000 Ex 8752
John Cazabon or Rosemary Ebling, Lead Biomedical Scientists
T 020 3299 1559
