Anti Tissue Transglutaminase antibodies
Immunology and Allergy Overview / Directory
Purpose of the test
The enzyme tissue transglutaminase is the target of autoantibodies found in the serum of patients with coeliac disease (CD). Anti-tTG antibodies of the IgA isotype are very sensitive and specific markers of CD. There are eight described isoforms of the enzyme tTG and it is isoforms 2 that is significant in CD. Isoform 3 appears to be the target in dermatitis herpetiformis. The ELISA based assay is compromised in IgA deficiency hence knowledge of the patient IgA status is recommended (we are able to offer IgA serum concentration if required). In cases of IgA deficiency we offer IgG anti-tTG. Due to its high sensitivity anti-tTG is a good screening test for CD but we routinely perform anti-endomysial antibodies on all positive anti-tTG sera due to the higher specificity of this assay and would recommend this algorithm to clients.
Sample Requirements
2 ml freshly separated serum
Storage and Transport
Serum stored at 4°CC should be sent by first class post
Turnaround Time
6 working days
Time Limit for Extra Tests
Five days
Factors affecting results or interpretation
All tests are compromised by prolonged transit times. For this reason, date of bleed and date of postage must be provided with each request.
Price
Price available on application - please contact adrianturner1@nhs.net. Discounts could be available for significant workloads.
Contacts
John Cazabon or Rosemary Ebling, Lead Biomedical Scientists
T 020 3299 1559
Dr Ted Davies Consultant Clinical Scientist
T 020 3299 9000 Ex 8752
