Anti Liver Cytosol 1 blot
Immunology and Allergy Overview / Directory
Purpose of the test
Anti-liver cytosol 1 (anti-LC 1) is associated with type 2 autoimmune hepatitis (AIH). The molecular target of anti-LC 1 is the enzyme formiminotransferase-cyclodeaminase. This autoantibody may be difficult to detect in conventional indirect immunofluorescence and is virtually impossible to detect in the presence of anti-liver kidney microsomal antibody 1, the more commonly seen marker of type 2 AIH. Anti-LC1 may occur in the absence of anti-LKM 1 (1) hence the need to identify it correctly is all the more important in establishing a diagnosis.
Sample Requirements
Serum 2.0 ml.
Storage and Transport
Samples may be stored at 4°C prior to despatch. The use of first class letter post without the need for coolant is sufficient.
Turnaround Time
Assay is performed weekly but samples with clinical urgency can be processed on the day of receipt. If this is required, a telephone call to this department prior to dispatch is essential.
Time Limit for Extra Tests
Five days
Factors affecting results or interpretation
All tests are compromised by prolonged transit times. For this reason, date of bleed and date of postage must be provided with each request.
Price
Refer to Autoimmune Liver Blot Screen test information sheet. Price available on application - please contact adrianturner1@nhs.net. Discounts could be available for significant workloads.
Contacts
Dr Ted Davies
T 020 3299 9000 Ext 8752
References
1. Features and outcome of autoimmune hepatitis type 2 presenting with isolated positivity for anti-liver cytosol antibody. Bridoux-Henno L et al
Clin Gastroenterol Hepatol. 2004 Sep; 2(9): 825-30.
