(i) Histological evaluation (tissue diagnosis) of adult and paediatric liver disorders

(ii) Activity grading and staging of liver disease

(iii) Cause of liver allograft dysfunction

N/A

Routine histology:

Immerse specimen in 10% formal saline in suitable specimen container and transport to the laboratory as soon as possible.

Non routine histology: (- e.g. suspected malignancy or metabolic disorder, tissue copper or iron estimation):

Please contact the laboratory when planning the biopsy to ensure that tissue is appropriately harvested / stored / fixed.

Case for opinion:

stained, unstained sections and / or paraffin block (to be returned to the referral hospital)

The tissue core should be immediately placed (left floating without blotting paper) in a labelled perspex container with 10% formal saline

Seal fixed tissue sample in specimen transport bag with sufficient absorbent material to contain the contents in case of leakage. Place in a padded box for transport to destination. Seal accompanying request form in a plastic bag to prevent contamination in case of leakage. Tape the box closed, label as Pathological Specimen and send by registered post or courier

Written reports (needle and small biopsy specimens) are usually available within one working day of receipt

2 to 4 working days for immunohistochemistry and / or in-situ hybridization techniques

i) Poor fixation, delayed immersion in formalin

ii) Failure to label pot with patient name, date of birth and precise identifier

iii) Failure to fill in a consultation-request form, in particular clinical information, source of biopsy and doctor to be notified of findings; with slides or paraffin block, failure to include copy of original (identifying) histopathology report or requisition

Prices available on application - please contact adrian.turner@kch.nhs.uk. Discounts could be available for significant workloads.

Section-Head: Professor Bernard Portmann

Consultant Histopathologist:

T 020 3299 3734

F 020 3299 3125

M 0797 965 5452

Laboratory: Anne Rayner, Chief Biomedical Scientist

T 020 3299 2237

http://referrals.kch.nhs.uk/directory/liver/liver-pathology/hepatitis-testing-hts/

Ciclosporin - LC/MS

Everolimus

HBV DNA (quantitative)

HCV genotyping

HCV RNA (qualitative)

HCV RNA (quantitative)

HDV antibody (IgM)

HDV antibody (Total)

HEV antibody (IgG)

HEV antibody (IgM)

Liver biopsy

Mycophenolate – LC/MS

Sirolimus - LC/MS

Tacrolimus – LC/MS